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Phis2.1

WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … Webb17 juni 2024 · Expression pattern analysis of OsbZIP62 under abiotic stresses and hormones. a, Relative expression level of OsbZIP62 under various stresses, including PEG6000 (15%), cold (4 °C), heat (42 °C), NaCl (150 mM), H 2 O 2 (1%) and ABA (100 μM). b, Main stress-related cis-acting elements in the OsbZIP62 promoter region.c, Relative …

Phis2 1 Bait Plasmid TaKaRa Bioz

Webb26 mars 2024 · The promoter sequence (1800 bp) of MdMATE8 (MDP0000175055) was inserted into the pHIS2.1 vector (Clontech, Palo Alto, CA, United States), which contained … Webb24 mars 2024 · In case of 4CRM1-pHis2.1, 4CRM1mut2-pHis2.1, and 4CRM1mut5-pHis2.1, 50 mM 3-amino-1,2,4-triazole (3-AT) was sufficient to suppress background growth of yeast colonies. DNA from the TF-only prey library in pDEST_GAD424 (Mitsuda et al. 2010) was transformed into Y1HGold containing 4CRM1-pHis2.1. inxs songs list. live never tear us apart https://mcneilllehman.com

pHIS2 [PVT4037] - $162.00

WebbBait Vector Phis2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > bait vector phis2. bait vector phis2 TaKaRa is a verified supplier TaKaRa ... Webb5 feb. 2024 · Either pGADT7-RrMYB18 or pGADT7-AtMYB46 was used to co-transform yeast strain Y187 with either the pHIS2.1-P RrCESAs or pHIS2.1-P AtCYCs reporter plasmids, respectively. DNA–protein interactions were quantified using the Matchmaker one-hybrid system (Clontech, now TaKaRa) according to the manufacturer’s instructions … Webb16 okt. 2014 · Specifically, the bait construct pHis2.1−2 × 150 was generated by cloning tandem copies of the 150-bp enhancer (2 × 150) into the MluI site of pHis2.1, a one … inxs song need you tonight

Overexpression of PvWOX3a in switchgrass promotes stem

Category:PT3705-5 (PR32049) pHIS2

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Phis2.1

Combinatorial requirement of W- and WT-boxes in microbe …

WebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for … Webb1 dec. 2024 · According to the hereditary mode of sterility genes, this vital agronomic trait can be further divided into cytoplasmic male sterility (CMS) and genic male sterility …

Phis2.1

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http://www.coolaber.com/usr/2160/File/F/F4671.pdf WebbpHIS-2 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available …

WebbThe promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs ... WebbThe target DNA sequence, or Bait Sequence, is cloned into pHIS2.1 as one copy or tandem repeats. Interaction between a DNA-binding protein and the target sequence stimulates …

Webb1 site: C C C A G C G G G T C G: After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility. AmpR 1973 .. 2833 = 861 bp 286 amino acids = 31.6 kDa 2 segments WebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the …

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WebbpHis2: 质粒类型: 酵母单杂交载体: 高拷贝/低拷贝:--启动子:--克隆方法: 多克隆位点，限制性内切酶: 载体大小: 7207bp: 5' 测序引物及序列:--3' 测序引物及序列:--载体标签: TRP1, HIS3: … inxs soundtrack songsWebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & Screening Kit (#K1617-1). inxs songs what you needWebb1 juni 2024 · When the insertion sequence is predicted as known cis-acting element , construct the pHIS2 reporter by three tandem copies of the studied elements inserted at … inxs spill the wineWebb+ 5 µg pHis2.1 20 µl Note: To denature carrier DNA, heat to 95–100°C for 5 min, then cool rapidly in an ice bath. NOTE NOTE Protocol No. PT3529-1 Version No. PR7Z2445 32 3. Add competent cells and gently mix. 4. Add PEG/LiAc and gently mix. 5. Incubate at 30°C . NOTE: Mix cells every 10–15 min. www.clontech.com 50 µl 500 µl 30 min 600 µl 2.5 ml on premises data gateway release notesWebbyeast reporter vector phis2 1 bd ( TaKaRa ) 95. TaKaRa yeast reporter vector phis2 1 bd. Yeast Reporter Vector Phis2 1 Bd, supplied by TaKaRa, used in various techniques. Bioz … on-premises data gateway log filesWebb12 okt. 2024 · Moreover, a yeast one-hybrid assay was performed to verify the interaction between CnGATA20 and the CnOLE18 promoter. pHIS2.1-pro53 and pGADT7-53 (pHIS2.1-pro53+pGADT7-53) were cotransferred into yeast Y187 as a positive control, and pHIS2.1-proCnOLE18+pGADT7-53 was used as a negative control. inxs song writerWebb6 feb. 2024 · Europe PMC is an archive of life sciences journal literature. on-premises data gateway licensing