Expression of ascorbate oxidase

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August undefined, 2024

WebJan 14, 2024 · The activities and expression of NADPH oxidase (NOX) and antioxidant enzymes in the corresponding samples were also determined. ... The total antioxidant capacity and the activities of antioxidant enzymes ascorbate peroxidase (APX), superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were measured using an … WebMar 20, 2024 · Ascorbate oxidase (AO) is a member of the multi-copper oxidase family of enzymes and converts ascorbate to monodehydroascorbate at the same time as reducing oxygen to water. The enzyme is present in the apoplastic space of plant cells and seems to be particularly highly expressed in roots and fruits of the Cucurbitaceae family.

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WebApr 9, 2024 · HIGHLIGHTS. who: Stefania Fortunato et al. from the Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, Bari, Italy have published the paper: Redox Signaling in Plant Heat Stress Response, in the Journal: Antioxidants 2024, 12, 605. of /2024/ SUMMARY WebJul 11, 2003 · We show that expression of AO is regulated by light and by plant hormones that modulate growth. Moreover, we demonstrate that enhanced apoplastic AO activities in transformed tobacco plants favor increased growth. Taken together, these observations provide evidence that AO has a role in the control of growth. RESULTS 効果測定初めて

cDNA cloning and gene expression of ascorbate oxidase in …

WebJan 26, 2024 · Ascorbic acid is considered to be one of the most important antioxidants in plants and plays a vital role in the adaptation of plants to unfavorable conditions. In the present study, an ascorbate oxidase gene (Solyc04g054690) was over-expressed in cherry tomato cv. West Virginia 106 lines and compared with previously studied RNAi silenced … WebAscorbate oxidase (AO) is a cell wall-localized enzyme that uses oxygen to catalyse the oxidation of ascorbate (AA) to the unstable radical monodehydroascorbate (MDHA) which rapidly disproportionates to yield dehydroascorbate (DHA) and AA, and thus contributes to the regulation of the AA redox state. In enzymology, a L-ascorbate oxidase (EC 1.10.3.3) is an enzyme that catalyzes the chemical reaction 2 L-ascorbate + O2 2 dehydroascorbate + 2 H2O Thus, the two substrates of this enzyme are L-ascorbate and O2, whereas its two products are dehydroascorbate and H2O. 効果測定何回で受かる

Effect of ascorbate oxidase over-expression on ascorbate …

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WebMar 25, 2024 · Thermographic correlation analysis showed that the correlation between relative expression and log2 fold change values at 24 h, 48 h, 72 h and 96 h were very high, with R2 values of 0.96, 0.90, 0. ... WebSep 21, 2006 · Ascorbate oxidase (AO, EC 1.10.3.3) is a member of the multicopper oxidases family. It catalyzes the oxidation of ascorbic acid (AA) to dehydroascorbic acid (DHA) via monodehydroascorbate (MDHA), with the concomitant reduction of molecular oxygen to water. In melon (Cucumis melo), ascorbate oxidase is encoded by a … au 池袋サンシャインWebgenes whose expression changes in response to high light (HL), including the H 2O 2 scavenger, ASCORBATE PER-OXIDASE 2 (APX2). We carried out a screen in Arabi-dopsis thaliana for lesions that alter HL-induced expression of APX2 to identify components in abiotic stress signalling pathways. High light was used as it can be instan- 効果測定勉強アプリ

"WebA thylakoidal APX (TAPX) gene was cloned from sugarcane leaves by using RT-PCR technique, and real-time fluorescence quantitative PCR analysis showed the TAPX gene expressed in the roots, stems and leaves of Sugarcane plant, and the highest expression level was found in the leaves. 8 References SHOWING 1-10 OF 20 REFERENCES … " - Expression of ascorbate oxidase

Expression of ascorbate oxidase

Expression of Ascorbic Acid Oxidase in Zucchini Squash

WebMar 7, 2024 · Further experiments revealed that VvBKI1 interacts with a cytoplasmic ascorbate peroxidase, VvAPX1, an ROS-scavenging protein. Transient expression of VvAPX1 in grape and N. benthamiana promoted its resistance against P. viticola, and P. capsici. Moreover, VvAPX1 transgenic Arabidopsis is more resistant to H. arabidopsidis. WebAscorbate peroxidase (APX), reported first time in horseradish plant (1979), is an important enzyme known for higher affinity for intracellular H2 O 2 than other types of peroxidases like catalases, peroxiredoxins in Leishmania and Trypanosoma parasites.

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WebThe ascorbate oxidase (AO) gene was another gene overexpressed in the calli of the three genotypes. However, the transcriptional profile of the other genes involved in ascorbate oxidation (i.e., the four ascorbate peroxidases, APXs) was complex, also because the three genotypes showed a different response to the tissue culture. WebMar 5, 2024 · In particular, four major expression patterns can be identified via the hierarchical clustering of the heat maps (absolute Pearson correlation, average linkage): the genes corresponding to...

WebAscorbate oxidase is a copper-containing enzyme found in melons, seeds, cereals, and fruits and vegetables. It can oxidize ascorbic acid to produce water and dehydroascorbic acid. Compared with non-enzymatic oxidation, ascorbic acid oxidase acts to generate water, while the former generates hydrogen peroxide. Figure 1. Ascorbate oxidase dimer. WebApr 26, 2024 · Ascorbate oxidase (AO) is a glycoprotein and a member of blue copper oxidase family that mediates ascorbate oxidation to dehydroascorbate (DHA) which usually follows high affinity transport across the membrane ( Smirnoff, 2000; Szarka et al., 2004; Barbehenn et al., 2008; De Tullio et al., 2013 ).

WebAscorbate oxidase is a homodimeric enzyme with a molecular mass of 70 kDa and 552 amino acid residues per subunit (zucchini). The three-domain structure and the location of the type-1 and trinuclear copper centers in the ascorbate oxidase monomer as derived from the crystal structure are shown in Figure 33. WebOxidoreductase that oxidizes ascorbic acid to dehydroascorbate. Application Use Ascorbate Oxidase in a variety of diagnostic tests to eliminate the interference of ascorbic acid, since ascorbic acid interferes with the Trinder reaction that is widely used for the colorimetric determination of analytes.

WebEctopic Gene Expression to Restore the Ascorbate Biosynthesis Pathway in Vertebrates Lacking Functional Gulo

WebSep 22, 2006 · Ascorbate oxidase (AO) is a cell wall-localized enzyme that uses oxygen to catalyse the oxidation of ascorbate (AA) to the unstable radical monodehydroascorbate (MDHA) which rapidly disproportionates to yield dehydroascorbate (DHA) and AA, and thus contributes to the regulation of the AA redox state. 効果測定卒検前ムサシWebSep 6, 2016 · As a member of a small multigene family of multicopper oxidases, ascorbate oxidase (AO; EC 1.10.3.3) catalyzes the oxidation of ascorbic acid (AA) to dehydroascorbate (DHA), thereby generating oxidative signals in apoplasts [6–8]. 効果測定卒検前受からないWebNov 27, 2010 · AO is a cell wall-localized enzyme that uses oxygen to catalyze the oxidation of ascorbate to the unstable MDHA which rapidly disproportionates to yield DHA and AsA, and thus contributes to the modulation of the AsA redox state and perception of external and internal stimuli of plant cells. 効果測定出やすい標識WebSep 1, 2011 · AO expression levels in transgenic lines were monitored by semi-quantitative reverse transcriptase-polymerase chain reaction. The decreased AO enzyme activity and significantly improved AsA... au 決済アップルWebMar 9, 2024 · Vitamin C, also known as ascorbic acid or formulated as ascorbate sodium salt, has been one of the mostly used agent in cancer therapies, especially to counteract oxidative stress. ... (IFN-γ)-induced dual oxidase 2 (Duox2) and dual oxidase A2 (DuoxA2) expression in human pancreatic cancer cell lines. J. Biol. Chem. 2011; … au 決済イオンWeb57 rows · Ascorbate peroxidase (APX, EC 1.11.1.11) is a heme-peroxidase enzyme that utilizes ascorbate as a substrate to decompose H 2 O 2 into water and … 効果測定勉強コツWebFeb 17, 2024 · An enzyme named ascorbate oxidase (AAO) is known to oxidize ascorbate and deleteriously affect the plant system in response to stress. Genome-wide analysis of ascorbate oxidase gene family has led to the identification of five, three, seven, four and six AAO genes in Oryza sativa, Arabidopsis, Glycine max, Zea mays and Sorghum bicolor … 効果測定勉強サイト